Hi Martin,
even with 127 slices at an image size of 953*856, the number of
slices is much smaller than the image dimensions. So you have a
rather thin object (as compared to the other dimensions) in the
center of a thick box (the box is roughly the size of the diagonal).
Thus, depth cueing will dim everything by roughly the same amount.
I have rescaled your large stack to 475*427 pixels, 400 slices. Now,
depth cueing becomes obvious (but the limited resolution in z
direction is obvious, too...)
Anyhow, a better depth cueing algorithm would be nice! Unfortunately,
the "3D project" code looks rather complex. Is anyone familiar with
that piece of code and could do this?
Michael
________________________________________________________________
On 30 Oct 2009, at 10:23, Martin Klingler wrote:
On Thu, October 29, 2009 12:40, Martin Klingler wrote:
> Hi,
>
> I use 3D project on fluorescent image stacks of basically hollow
> structures. It works very well except
> one feature: I tried different settings for interior depth cueing and
> surface depth cueing - never could
> I detect any effect of these settings, structures close to top and
> structures close to bottom of the
> stack always have the same intensity.
>
> What I would like to achieve is that structures close to the bottom
> (i.e.
> "on the other side of the
> object" would be projected less bright than those on top (near the
> viewers
> eye).
>
> any idea what the problem might be?
>
> Martin
>
Locked
