substract line slop in a direction.

[Lee S]

Dear,

I would like to process the sloped image (Z value) to get a flatten image
I found the substract backgroud, but it seems to manipulate an image using
circular mask.

How do I get the flattened (X, Y, XY or 1st/2nd order global plane fit)
image using ImageJ?

Best,

Lee

[Joachim Wesner]

Hi,

I don´t think Imagej can do that "as is"!?

To be sure, you mean you have an image z(x,y) and you want to find and/or
also subtract the best fit plane zfit(x,y) = x*a + y*b + c from it?

I have a plugin that can do this, it also takes a second order (parabolic!
What is a 2nd oder plane?) fit into account if the user wishes to.

Joachim Wesner



                                                                           
             Lee S                                                        
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             03.12.2009 22:42                                              
                                                                           
                                                                           
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Dear,

I would like to process the sloped image (Z value) to get a flatten image
I found the substract backgroud, but it seems to manipulate an image using
circular mask.

How do I get the flattened (X, Y, XY or 1st/2nd order global plane fit)
image using ImageJ?

Best,

Lee



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[Michael Schmid]

Hi Lee,

if I understand it correctly, you want to subtract a polynomial fit.  
There is a plugin for this:
   http://imagejdocu.tudor.lu/doku.php?
id=plugin:filter:fit_polynomial:start

Michael
________________________________________________________________

On 3 Dec 2009, at 22:42, Lee S wrote:

> Dear,
>
> I would like to process the sloped image (Z value) to get a flatten  
> image
> I found the substract backgroud, but it seems to manipulate an  
> image using
> circular mask.
>
> How do I get the flattened (X, Y, XY or 1st/2nd order global plane  
> fit)
> image using ImageJ?
>
> Best,
>
> Lee

[Gabriel Landini]

On Friday 04 Dec 2009  09:24:57 you wrote:
> if I understand it correctly, you want to subtract a polynomial fit.  
> There is a plugin for this:
>    http://imagejdocu.tudor.lu/doku.php?
> id=plugin:filter:fit_polynomial:start

Furthermore, if your image is made by *transmitted* light (or electrons or
whatever), the background correction is not made through subtraction, but via
the ratio of the image and the background.
Here are some examples:

http://imagejdocu.tudor.lu/doku.php?id=howto:working:how_to_correct_background_illumination_in_brightfield_microscopy

Cheers

G.