successive measurements of growth annuli on an established trasect

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successive measurements of growth annuli on an established trasect

Mark Schmitz
I'm utilizing cross-sectioned dorsal fish spines to back-calculate length at age
of walleye.  Essentially I am looking for advice on how to measure the distance
between successive growth annuli (similar to growth rings on a tree).  I'm
attempting to 1) establish a transect from the origin (center) of the spine to
the outside edge followed by 2) along this transect, mark the intersection of
numerous, yet successive, growth rings which would allow me to 3) record the
relative distance between these points that would allow me to back-calculate
length at different known ages.  The key unknown issue is how to force these
points of measurement on an established transect w/o loosing that transect
after every measurement.  Any thoughts?

Mark Schmitz
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Re: successive measurements of growth annuli on an established trasect

Sullivan, Michael J (College of Med.)
My quick thought: use a line ROI and then plot the profile.  "Analyze
--> Plot Profile"  you can then save the values from the plot as a text
file and process it for your measurements. --- Mike

-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of
Mark Schmitz
Sent: Friday, October 14, 2005 11:34 AM
To: [hidden email]
Subject: successive measurements of growth annuli on an established
trasect

I'm utilizing cross-sectioned dorsal fish spines to back-calculate
length at age of walleye.  Essentially I am looking for advice on how to
measure the distance between successive growth annuli (similar to growth
rings on a tree).  I'm attempting to 1) establish a transect from the
origin (center) of the spine to the outside edge followed by 2) along
this transect, mark the intersection of numerous, yet successive, growth
rings which would allow me to 3) record the relative distance between
these points that would allow me to back-calculate length at different
known ages.  The key unknown issue is how to force these points of
measurement on an established transect w/o loosing that transect after
every measurement.  Any thoughts?

Mark Schmitz