Re: Combining image / panoramic

Posted by GloryField on
URL: http://imagej.273.s1.nabble.com/Combining-image-panoramic-tp3682962p3682966.html

Another question about using the analyze particles in ImageJ/FIJI

here's a sample image I made using stitching:
http://www.swapdrive.com/file.asp?ID=tG+4c+dQUV2uaBUzGE9QXIqvYD6NpPr0zU3HMp4maEzHuo55/04pqQ==url

I'm trying to measure the average area of each of the axons within the nerve, including the myelin sheath.

What I did so far is:
Process>Subtract Background>5 pixels
Process>Binary>Make Binary
Analyze>Analyze Particles>Size 4-350, circularity 0.25-1.0, show outlines, include holes

I chose 350 as max size because that allows me to eliminate blood vessels within the nerve.
I arbitrarily chose 0.25 circularity because I didn't know what else to pick.

Doing this gave me an average size of 50.668 pixels.  I will put a slide with a length marker under microscope with same magnification so I can use "Analyze > Set Scale" and get values in micrometers.

Do you guys think this would be an OK way of measuring the axon cross section area?

Thank you again!