Converting 32-bit to 8 bit images

Posted by Dr Adam Cliffe on Sep 27, 2011; 3:22am
URL: http://imagej.273.s1.nabble.com/Converting-32-bit-to-8-bit-images-tp3683067.html

Hi Everyone,

I'm trying to do some spectral unmixing using Joachim Walter's Spectral Unmixing plugin. I can get it to work fine, and at the end it produces 2 32-bit stacks. My problem is converting these back to 8-bit images.  The 32-bit image has a range of -330.8  to +577.82. The range I'd like to keep in the 8-bit image is from say -40 to +353. (as seen by adjusting the max/min range.

I've tried setting this range, then hitting 'Apply' in the Brightness/contrast window. Whatever I do, when I convert to 8-bit, I get the same image as if I convert the original 32-bit image.

Can anyone tell me where I'm going wrong or offer any good advice for converting 32-bit to 8-bit?

thanks

Adam
 


Dr Adam Cliffe
Research Scientist, Rorth Lab

Institute of Molecular and Cell Biology
61 Biopolis Drive
Proteos
Singapore 138673

tel: [phone]