Posted by
John Oreopoulos on
URL: http://imagej.273.s1.nabble.com/Measuring-distance-between-two-points-tp3683081p3683087.html
Hi Liliana,
First, I'm assuming you're talking about measuring the distance
between two points in 3D space that have been captured in a single
time point 3D stack and not a time-lapse 2D stack. A simple way to do
this is to draw a line on one image of the stack that would intersect
both spots of interest if they both occurred in the same image (You
could add all images together to make this happen, draw the line, and
then draw it again on the original stack). Then click Image->stacks-
>reslice. This produces a new image that is a cross-section side on
view of the stack along the line you drew, and you should now see
both spots separated by some vertical and horizontal pixel distance.
Now you need to know your lateral and axial pixel dimensions in order
to calculate the distance. However, I'm not exactly sure how ImageJ
draws reslice images from lines that were drawn on a diagonal. You
might have to consider that when calculating the lateral distance.
I'm sure someone out there has already produced a much better ImageJ
plugin would essentially do all of this for you by clicking on the
two points of interest in the stack, but you may have to hunt around
on the plugins part of the website to find that. Can anyone else
recommend a plugin?
John Oreopoulos
On 20-Jun-08, at 11:07 AM, Liliana de Jesus Duarte Ferreira wrote:
> Dear all
>
> Can anyone tell me how to measure the distance between two spots
> that are
> in different sections of the same stack?
>
> Thanks
>
> Liliana
>
>
>
>
>
>
>
> --
> Liliana Ferreira
> Plant Genetic Engineering Laboratory
> Instituto de Tecnologia Química e Biológica
> Av. Republica, Apartado 127
> 2781-901 Oeiras
> Portugal
>
>
[hidden email]
> phone: +351 214469648