Re: 2D gaussian fit of puncta

Posted by dpoburko on
URL: http://imagej.273.s1.nabble.com/Re-2D-gaussian-fit-of-puncta-tp3683281.html

Hi All,

   Has anyone has any success in implementing a 2-D Gaussian fit in
ImageJ? I am looking for a method of localizing the center and FWHM of
fluorescent puncta by implementing a simultaneous 2-D fit. Some
colleagues have suggested that this would be trivial in MatLab, but it
seems like this would be a valuable tool to be able to run in ImageJ
(independent of other programs). I am currently using Adrian's FWHM.
It's quite good, but I would like to go beyond the independent X and Y
fits. If anyone has this implemented or has run into pitfalls in past
attempts, I'd love to hear about it.

Cheers,
Damon

--
Post Doctoral Fellow
Tsien Lab, Molecular&  Cellular Physiology
Stanford University