Re: Leveling Image

Posted by ashamim on Aug 23, 2011; 9:20am
URL: http://imagej.273.s1.nabble.com/Leveling-Image-tp3683357p3683366.html

Gabriel

Thanks for the reply. Actually I am myself little bit confused about the same thing that how will it differentiate between the actual data and the artifact. And how much actual data will get lost in following such an approach.

I have already removed the non uniform background from the original image by fitting a plane on it and subtracting from the original image. I have attached the sample image. The image on the left most is what I get from AFM and the image in the middle is what I get after correcting the background and the image on the right is my target. There are even many plugins available to perform such an operation. Now i am looking for some algorithm to bring the image to the same level. One approach is by subtracting the median from each line individually but i couldn't find much reading material about it that could explain why will it not remove the actual data and only the artifacts.

i will just try to subtract the median same way u mentioned and will let u know if i have some positive results. May be for that I have to modify the median filter to some extent.

Thanks Again
Ahsan