use skeletonized channel to analyze 2nd channel

Posted by Michel Herde on Aug 11, 2011; 4:25am
URL: http://imagej.273.s1.nabble.com/use-skeletonized-channel-to-analyze-2nd-channel-tp3683536.html

Dear all,
I want to analyze synaptic input onto a dendrite and have confocal image stacks with 2 channels, one with dendritic label and the other one labeling presynaptic boutons.
My idea was to skeletonize the dendritic channel and then define a specific distance from the skeleton in which puncta in the synaptic channel can be counted.
I managed to create a skeleton of the dendrite, however I'm stuck with further steps.

Can I automatically create a selection at defined distance from the skeleton which I could then use on the synaptic channel?
Or draw around the skeleton and then do an arithmetic subtraction from the synaptic channel?

Any ideas welcome!

Cheers

Michel