Re: Measure intensities *on* rather than within an ROI line, and be able to specify the "line" width.

Posted by ctrueden on Aug 03, 2011; 2:46pm
URL: http://imagej.273.s1.nabble.com/Measure-intensities-on-rather-than-within-an-ROI-line-and-be-able-to-specify-the-line-width-tp3683556p3683563.html

Hi Emmanuel,

You can transform your selection into a boundary of designated width using
Edit > Selection > Make Band. Will that do the trick?

-Curtis

On Wed, Aug 3, 2011 at 9:24 AM, Emmanuel Levy <[hidden email]>wrote:

> Hi,
>
> I would like to measure pixel intensities along the border of
> particles (cell membranes). In my macro each particle has a
> corresponding ROI. That would be equivalent to being able to measure
> the intensity *on* (and not within the ROI line), and be able to
> specify the line width (e.g., 3-15 pixels).
>
> In principle this looks simple but I'm not sure what would be the best
> way to achieve it. The simplest (and very rough) way I thought of was
> to run every measure twice, one on the "dilated" particles and one on
> the "eroded" same particles. However, it's not optimal if I want to
> get measures for different widths simultaneously (e.g., 3,5,7,9,11,13
> and 15 pixels "ribbon" width).
>
> Thanks for any advice you could give!
> Best,
>
> Emmanuel
>