> Hi everyone,
>
> This is my first time to try to write a ImageJ micro, so sorry for the easy
> question I will ask,
>
> The situation is that I have a plate within which there are 8 wells (4 wells
> in one row, 2 rows together, in the attachment you can find one pic showing
> this pattern), one fruit fly is moving around in each well. I recorded these
> 8 flies at a certain frequency within observation time, so I got a bunch of
> images in the end (the only thing moving is flies) . I want to crop each
> well from the whole plate picture, then run the object tracking plugin to
> check locomotion of this fly. Since the pattern of 8 wells is constant,
> instead of manually cropping each well, I hope I can write a macro to
> "pattern crop" the 8 wells at the same time.
>
> My main logic is by changing the x and y value of "makeRectangle" to crop
> consequential wells. (below is two trials hoping to crop the 4 wells in the
> first row, after success I would go on to add the second row)
>
> *I tried first the most straight-forward way, and it works:*
>
> run("Image Sequence...", "open=[C:\\Documents and Settings\\WangLab\\Desktop\\20110628D\\ZZ20100628D100] number=20 starting=1 increment=1 scale=100 file=[] or=[] sort");
>
>
> makeRectangle(60+100*0, 120, 200, 200);
>
> run("Duplicate...", "title=0 duplicate range=3-20");
>
> saveAs("tiff", "C:\\Documents and Settings\\Wang Lab\\Desktop\\TRY ZZ\\0");
>
> run("Close");
>
> makeRectangle(60+100*1, 120, 200, 200);
>
> run("Duplicate...", "title=1 duplicate range=3-20");
>
> saveAs("tiff", "C:\\Documents and Settings\\Wang Lab\\Desktop\\TRY ZZ\\1");
>
> run("Close");
>
> makeRectangle(60+100*2, 120, 200, 200);
>
> run("Duplicate...", "title=2 duplicate range=3-20");
>
> saveAs("tiff", "C:\\Documents and Settings\\Wang Lab\\Desktop\\TRY ZZ\\2");
>
> run("Close");
>
> makeRectangle(60+100*3, 120, 200, 200);
>
> run("Duplicate...", "title=3 duplicate range=3-20");
>
> saveAs("tiff", "C:\\Documents and Settings\\Wang Lab\\Desktop\\TRY ZZ\\3");
>
> run("Close");
>
>
> *Then I used "for" loop as follows:*
>
> run("Image Sequence...", "open=[C:\\Documents and Settings\\WangLab\\Desktop\\20110628D\\ZZ20100628D100] number=20 starting=1 increment=1
> scale=100 file=[] or=[] sort");
> for (i=0; i<4; i++) {
> makeRectangle(60+100*i, 120, 200, 200);
> run("Duplicate...", "title="+i+" duplicate range=3-20");
> saveAs("tiff", "C:\\Documents and Settings\\WangLab\\Desktop\\TRYZZ\\"+i+");
> run("close");
> }
>
> After I run this macro, an error pop out:
> ")" expected in line 6
> <run>("close");
>
> How can I correct this? Anyone can help me? Thanks a lot in advance!
>
> Zheng
>
>
> --
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>