Automatic ROI for particle anamysis

Posted by samuel-co on Jul 28, 2011; 10:53am
URL: http://imagej.273.s1.nabble.com/Automatic-ROI-for-particle-anamysis-tp3683626.html

Hi,

I have RGB pictures from nucleus staining and I would like to measure
the ratio of red and green staining in each nucleus. My way of doing it
so far was to draw manually a ROI around each nucleus and measure the
intensity with /analyse/measure/.

My question is : is there a plugin/macro to automatically creat ROI
around all nucleus (I have thought to threeshold my picture, apply a
granulometric filter and import this new picture as an ROI, but I
couldn't found any way to do that) but with independant ROI for each
nucleus, to be able to have a measure for each?


Thanks,
Samuel

--
Samuel Collombet
[hidden email]
237 B/6 Gilmerton Road, EH16 5TH Edinburgh