http://imagej.273.s1.nabble.com/Automatic-ROI-for-particle-anamysis-tp3683626p3683628.html
> Hi Samuel,
>
> Here is the way I would do it.
>
> - Take your image of the nucleus and threshold it (if the SNR is low you can first apply a mean or gaussian filter).
> - On the thresholded image run Analyze-->Analyze Particles...
> - Check "Add to Manager"
>
> This will add every nucleus as a single ROI to the roi manager. Now you can measure the intensities of the ROIs individual or all at once in the red and the green channel.
>
> I hope that helps.
>
> Cheers Arne
>
>> -----Original Message-----
>> From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of
>> samuel collombet
>> Sent: jeudi 28 juillet 2011 12:53
>> To:
[hidden email]
>> Subject: Automatic ROI for particle anamysis
>>
>> Hi,
>>
>> I have RGB pictures from nucleus staining and I would like to measure
>> the ratio of red and green staining in each nucleus. My way of doing it
>> so far was to draw manually a ROI around each nucleus and measure the
>> intensity with /analyse/measure/.
>>
>> My question is : is there a plugin/macro to automatically creat ROI
>> around all nucleus (I have thought to threeshold my picture, apply a
>> granulometric filter and import this new picture as an ROI, but I
>> couldn't found any way to do that) but with independant ROI for each
>> nucleus, to be able to have a measure for each?
>>
>>
>> Thanks,
>> Samuel
>>
>> --
>> Samuel Collombet
>>
[hidden email]
>> 237 B/6 Gilmerton Road, EH16 5TH Edinburgh