Re: Kymograph analysis

Posted by Arne Seitz on Aug 02, 2011; 7:12pm
URL: http://imagej.273.s1.nabble.com/Kymograph-analysis-tp3683640p3683641.html

Dear Dinesh,

a kymograph is a very versatile tool to measure the speed of moving structures.

To measure the directionality of the movement a kymograph is less well suited. It is possible but you will have to define rules how you to draw the line. In the case of a cell you can e.g. say to always draw the lines from the nucleus to the cell periphery. By doing so you can distinguish between retrograde and anterograde transport.

To my opinion a kymograph is not at all suited to measure the total number of moving structures. But maybe I have not completely understood what you are planning to do.

More details could be maybe helpful.

Cheers Arne

> -----Original Message-----
> From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of
> dcjoshi
> Sent: mardi 2 août 2011 17:27
> To: [hidden email]
> Subject: Kymograph analysis
>
> Hi all
> I am very new to image J and have a question about analysis of
> kymograph. I
> understood the concept of anterograde and retrograde direction of
> mobility.
> As mobile mitochondria makes diagonal lines on kymograph so how do we
> count
> total number of mobile mitochindria in a kymograph obtained using time
> series program ?
>
> Dinesh C Joshi
>
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