Posted by
Cammer, Michael on
Aug 02, 2011; 7:35pm
URL: http://imagej.273.s1.nabble.com/Kymograph-analysis-tp3683640p3683643.html
If you treat a time series as a volume and use volume rendering, you often can see how the tracks interact and get a (rough) count of particles and paths.
-Michael
-----Original Message-----
From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of Seitz Arne
Sent: Tuesday, August 02, 2011 3:12 PM
To:
[hidden email]
Subject: Re: Kymograph analysis
Dear Dinesh,
a kymograph is a very versatile tool to measure the speed of moving structures.
To measure the directionality of the movement a kymograph is less well suited. It is possible but you will have to define rules how you to draw the line. In the case of a cell you can e.g. say to always draw the lines from the nucleus to the cell periphery. By doing so you can distinguish between retrograde and anterograde transport.
To my opinion a kymograph is not at all suited to measure the total number of moving structures. But maybe I have not completely understood what you are planning to do.
More details could be maybe helpful.
Cheers Arne
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