Re: Three contrast levels in stack
Posted by
Jan Eglinger-3 on
Jul 24, 2011; 6:26pm
URL: http://imagej.273.s1.nabble.com/Three-contrast-levels-in-stack-tp3683738p3683739.html
Hi,
On 22.07.2011 3:08 AM, gssjr wrote:
> I have a stack of hundreds of images (x,y,time) acquired from a microscope.
> Ideally there should only be three levels of contrast: hi, mid, lo (or
> -1,0,1 etc however you prefer)
>
> [...]
>
> If I look at a histogram for any given slice, I can clearly see three
> Gaussian-like peaks, but the positions and shapes of those peaks change
> every frame.
>
> Knowing that there should only be three states, how can I best normalize the
> stack so I can properly track each pixel?
Have you tried to use the IsoData Classifier? It might be of use in your
case.
http://fiji.sc/IsoData_ClassifierCheers,
Jan