Analyze Particles- saving zero results values
Posted by Citron, Bruce on Jun 03, 2011; 3:40pm
URL: http://imagej.273.s1.nabble.com/doFit-functions-problem-tp3683803p3683805.html
I have a macro that uses Analyze Particles and automatically increments
through a collection of pairs of tifs of dual stained cells, e.g., Hoechst
plus Annexin stained.
It works fine unless there are zeros counts in a particular tif. That data,
the 0 cell count, does not appear in the Summary window.
That makes it difficult for copy pasting into the data analysis excel
spreadsheet.
An example of part of a Summary table is:
Slice Count Total Area Average Size Area Fraction
Mean
HT22 MI H Well 2-2.tif 62 0.189 0.003 0.3 63.808 0.914
0.854
HT22 MI A Well 2-2.tif 9 0.035 0.004 0.1 87.602 0.948
0.887
HT22 MI H Well 2-3.tif 55 0.217 0.004 0.4 64.776 0.888
0.858
HT22 MI H Well 2-4.tif 95 0.466 0.005 0.8 73.232 0.914
0.881
HT22 MI A Well 2-4.tif 11 0.064 0.006 0.1 96.743 0.949
0.890
Where Well 2, field 3 did not have any Annexin positive cells and results in
a missing row from the data.
What would be a recommended way to obtain all of the rows of data, even if
there are zero cells (particles) present?
(And I am not wedded to the Summary table, I wouldn¹t mind writing data to a
file from the macro, if needed.)
Thank you,
Bruce
--
Bruce A. Citron, Ph.D., Bay Pines VA