Re: Stack Histogram is great!

Posted by Citron, Bruce on Jul 20, 2011; 6:11pm
URL: http://imagej.273.s1.nabble.com/doFit-functions-problem-tp3683803p3683807.html

I greatly appreciate adding the Stack Histogram option to
Adjust-Threshold!!!
Coincidentally, I needed this for the first time today, attempting to
process some extracted stacks from Olympus .oib confocal images.

For some reason, I was sporadically getting different abilities to threshold
at all for different stacks, likely due to first slice sometimes being
totally blank.
I was resorting to running the full histogram on the stack, multiplying by
the total voxels, then dividing by the voxel count above the desired level
to get some information about intensity in a 3-d region of interest.

I was running 1.45i, I googled ImageJ threshold stack and the third hit was
ImageJ News- Added a "Stack histogram" checkbox to the
Image>Adjust>Threshold dialog so I updated and all is right with the world.

Now, with the Stack Histogram option, I get exactly the same average
intensities to four significant digits by the histogram after thresholding
with Set Measurements- Limit to threshold.
Thanks very much,
Bruce