Re: analyzing a line, please

Posted by dscho on Jul 08, 2011; 5:24pm
URL: http://imagej.273.s1.nabble.com/analyzing-a-line-please-tp3683973p3683974.html

Hi Jimena,

On Fri, 8 Jul 2011, Jimena Berni wrote:

> I am trying to analyze the track of two different animals (image
> attached). One of them, the treatment is going kind of in circles while
> the other perform straight lines and then turns.
>
> I thought i could draw a segmented line over the track and get the value
> of angle between two adjacent points. I don't find a way of doing this.
> I thought that another alternative would be to get the XY information of
> the line. Is there a way of doing that?

Of course that is one way to do it, although the analysis would
definitely require programming a macro.

But if all you seek is to have a robust discrimination between treated vs
untreated animals, I think it might be better to measure enclosed area vs
average intensity. At least to me it looks like the "cuddled" paths cover
a smaller area, so the average brightness in that area is much higher than
in the other case.

Ciao,
Johannes