Re: analyzing a line, please

Posted by chai_san on Jul 09, 2011; 9:35am
URL: http://imagej.273.s1.nabble.com/analyzing-a-line-please-tp3683973p3683977.html

Hi Jimena,
Alternatively a skeletonization routine will give you path lengths and all sorts of neat parameters- directed or not, random or not etc. I remember seeing some plugins for this.
Cheers
Chaitanya


On 08-Jul-2011, at 10:54 PM, Johannes Schindelin wrote:

> Hi Jimena,
>
> On Fri, 8 Jul 2011, Jimena Berni wrote:
>
>> I am trying to analyze the track of two different animals (image
>> attached). One of them, the treatment is going kind of in circles while
>> the other perform straight lines and then turns.
>>
>> I thought i could draw a segmented line over the track and get the value
>> of angle between two adjacent points. I don't find a way of doing this.
>> I thought that another alternative would be to get the XY information of
>> the line. Is there a way of doing that?
>
> Of course that is one way to do it, although the analysis would
> definitely require programming a macro.
>
> But if all you seek is to have a robust discrimination between treated vs
> untreated animals, I think it might be better to measure enclosed area vs
> average intensity. At least to me it looks like the "cuddled" paths cover
> a smaller area, so the average brightness in that area is much higher than
> in the other case.
>
> Ciao,
> Johannes

------------------------------------------
Chaitanya Athale Dr. rer. nat.
Asst. Prof.
Biology
IISER Pune
www.iiserpune.ac.in/~cathale
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