Re: analyzing a line, please

Posted by dscho on Jul 09, 2011; 1:54pm
URL: http://imagej.273.s1.nabble.com/analyzing-a-line-please-tp3683973p3683978.html

Hi,

On Sat, 9 Jul 2011, Chaitanya Athale wrote:

> Alternatively a skeletonization routine will give you path lengths and
> all sorts of neat parameters- directed or not, random or not etc. I
> remember seeing some plugins for this.

As far as I saw, the paths were overlapping. If you have a skeletonization
routine that handles such paths correctly, I would be _very_ interested!

Thanks,
Johannes

> On 08-Jul-2011, at 10:54 PM, Johannes Schindelin wrote:
>
> > Hi Jimena,
> >
> > On Fri, 8 Jul 2011, Jimena Berni wrote:
> >
> >> I am trying to analyze the track of two different animals (image
> >> attached). One of them, the treatment is going kind of in circles while
> >> the other perform straight lines and then turns.
> >>
> >> I thought i could draw a segmented line over the track and get the value
> >> of angle between two adjacent points. I don't find a way of doing this.
> >> I thought that another alternative would be to get the XY information of
> >> the line. Is there a way of doing that?
> >
> > Of course that is one way to do it, although the analysis would
> > definitely require programming a macro.
> >
> > But if all you seek is to have a robust discrimination between treated vs
> > untreated animals, I think it might be better to measure enclosed area vs
> > average intensity. At least to me it looks like the "cuddled" paths cover
> > a smaller area, so the average brightness in that area is much higher than
> > in the other case.
> >
> > Ciao,
> > Johannes
>
> ------------------------------------------
> Chaitanya Athale Dr. rer. nat.
> Asst. Prof.
> Biology
> IISER Pune
> www.iiserpune.ac.in/~cathale
> ------------------------------------------
>