Re: Measure Colored Areas

Posted by Daniel James White on Jun 17, 2011; 8:47am
URL: http://imagej.273.s1.nabble.com/Measure-Colored-Areas-tp3684210p3684211.html

Hi Tina,


On Jun 17, 2011, at 6:02 AM, IMAGEJ automatic digest system wrote:

>
> Date:    Thu, 16 Jun 2011 15:17:14 -0400
> From:    Tina Safavie <[hidden email]>
> Subject: Measure Colored Areas
>
> I have an image that shows the antigen staining. The antigen is stained by a
> brown color, and I would like to know how to calculate the percentage or
> total amount of brown spots (which are goblet cells) that are present in the
> image. My first step, which is creating the image into a Binary image, skews
> and blends the brown and blue (cell membrane) areas, so I'm not getting an
> accurate reading on these values of Goblet cells. Neither the user guide nor
> tutorial seems to shed light on this problem, does anyone have any
> suggestions?
>
> Thanks.

you need to seaprate the 2 stains by color deconvolution,
see

http://www.dentistry.bham.ac.uk/landinig/software/cdeconv/cdeconv.html

Gabriel did a nice job there of explaining it.

cheers

Dan

>

Dr. Daniel James White BSc. (Hons.) PhD
Senior Microscopist / Image Visualisation, Processing and Analysis
Light Microscopy and Image Processing Facilities
Max Planck Institute of Molecular Cell Biology and Genetics
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