Re: Opening Mosaix zvi files with Bio-Formats

Posted by Jan Eglinger-3 on Jun 15, 2011; 1:16pm
URL: http://imagej.273.s1.nabble.com/Opening-Mosaix-zvi-files-with-Bio-Formats-tp3684230p3684232.html

Hi again,

On 15.06.2011 2:47 PM, Jan Eglinger wrote:
> In my case, the image data contains 90% black area and only very few
> elongated objects. That's why I have to rely on a good alignment of
> camera and stage. In my experience, stitching algorithms don't help much
> in that case, and I simply want to put the tiles together with 0% overlap.
>
> How can I achieve this with the current plugin?

Please excuse my slightly premature question. I now found that I can
open the mosaic file using "Stitch Multiple Series or Tile Scan File",
then unchecking "Compute overlap", setting the overlap to 0% and the
Fusion Method to "None".

However, this opens my 2-channel stack in RGB mode, thereby consuming an
unnecessary amount of memory.

Any hint how I can directly open as 2-channel hyperstack?


In my opinion, it would be nice to have an intuitive way to open this
kind of zvi file just as the acquisition software, i.e. AxioVision, does.

Cheers,
Jan