Re: Multi-Frequency Analysis in FIJI

Posted by dscho on Jun 07, 2011; 11:06pm
URL: http://imagej.273.s1.nabble.com/Multi-Frequency-Analysis-in-FIJI-tp3684332p3684336.html

Hi,

On Tue, 7 Jun 2011, Curtis Rueden wrote:

> ImageJ 2.0 (http://imagejdev.org/), due out in October, will feature an
> N-dimensional data engine. However, to work with multi-frequency data,
> more is required:
>
> First you need a file reader that can import the data successfully.
> Maybe Bio-Formats can (http://loci.wisc.edu/bio-formats), depending on
> your file format.
>
> After that you need an analysis algorithm to process your
> multi-frequency data. It is unlikely that you can do much naive image
> processing on FLIM data, and I do not know of any ImageJ plugins for
> multi-frequency data specifically, but you could write one.
> Unfortunately I cannot really comment further because we have not worked
> with that sort of FLIM data in my group (we do TCSPC).

Or in short: there was not enough information to answer the concrete
question thoroughly.

So: please provide more details of the project (example images would be
nice, and also the information what hypothesis underlies your experiment).

Ciao,
Johannes