Re: counting cells in confocal images

Posted by Citron, Bruce on Jun 07, 2011; 9:15pm
URL: http://imagej.273.s1.nabble.com/counting-cells-in-confocal-images-tp3684340p3684343.html

Leon-
> I hope I get it correctly that you suggest not splitting the channels for
> threshold (low\mid\high pos cells), but let particle analysis measure
> intensity and sort it out in excell?
€ Yes

> for particle analysis, is the range
> 70-400 and 0.8-1.0 circularity a standard setting you use?is this a setting
> that accepts like 95-99% of all normal cells? range is (i guess) dependant
> of the size of your image as well, right?
€ You just have to play with it and the area range may be square pixels, so
be careful.  For that and circularity, just analyze some of your images with
some of the smallest, largest, and most out of round cells that you would
count and make sure the range counts them and not other things.
-Bruce