Re: lamp temperature

Posted by Joel Sheffield on Jun 02, 2011; 6:40pm
URL: http://imagej.273.s1.nabble.com/lamp-temperature-tp3684358p3684362.html

At the risk of blowing my own, old horn, plates 20-21 of a powerpoint
tutorial I presented at the Microscopy Meetings in '08 disscuss the issue of
white balance, and provide a manual way of doing it.
http://imagej.nih.gov/ij/docs/examples/IJ-M&M08.ppt  I am not aware of a
plugin or macro for that purpose, although it should be relatively easy to
implement.

Joel


On Thu, Jun 2, 2011 at 2:04 PM, Kathleen McMillan <
[hidden email]> wrote:

> Hello- I am looking for advice on whether I can correct microscope images
> for variations in lamp filament temperature when analyzing slides for stain
> intensity. In a first batch of slides, the background was white, the
> (unstained) tissue was tan and the stain blue.  Using the Landini
> thresholding plugin to select for blue hues followed by measuring the mean
> saturation worked well.  However, in a second batch of slides - which were
> intended to be combined with the first in the analysis - the slide
> background and the unstained tissue are a yellowish color and the stain hues
> are in the blue-magenta region.  That creates a problem in separating the
> tissue from the background, and also the mean saturation of the hues
> attributable to the stain are different from the first batch.  Is there a
> good way to correct for lighting variation in these two image sets, that
> would allow an accurate and consistent stain intensity to be quantified?
>
> Thanks for any advice!
>

--


Joel B. Sheffield, Ph.D
Department of Biology
Temple University
Philadelphia, PA 19122
Voice: 215 204 8839
e-mail: [hidden email]
URL:  http://astro.temple.edu/~jbs