Posted by
Richard Cole on
Jun 02, 2011; 6:38pm
URL: http://imagej.273.s1.nabble.com/lamp-temperature-tp3684358p3684363.html
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Rich
Richard Cole
Research Scientist V
Director: Advanced Light Microscopy Core Unit
Wadsworth Center
Research Assistant Professor
Dept. of Biomedical Sciences
School of Public Health State University of New York
P.O. Box 509 Albany N.Y. 12201-0509
518-474-7048 Phone
518-474-4430 Fax
Email
[hidden email]
Website www.wadsworth.org/cores/alm/index.htm
-----Original Message-----
From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of Kathleen McMillan
Sent: Thursday, June 02, 2011 2:04 PM
To:
[hidden email]
Subject: lamp temperature
Hello- I am looking for advice on whether I can correct microscope images for variations in lamp filament temperature when analyzing slides for stain intensity. In a first batch of slides, the background was white, the (unstained) tissue was tan and the stain blue. Using the Landini thresholding plugin to select for blue hues followed by measuring the mean saturation worked well. However, in a second batch of slides - which were intended to be combined with the first in the analysis - the slide background and the unstained tissue are a yellowish color and the stain hues are in the blue-magenta region. That creates a problem in separating the tissue from the background, and also the mean saturation of the hues attributable to the stain are different from the first batch. Is there a good way to correct for lighting variation in these two image sets, that would allow an accurate and consistent stain intensity to be quantified?
Thanks for any advice!
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