Posted by
ctrueden on
May 27, 2011; 8:44pm
URL: http://imagej.273.s1.nabble.com/multi-channel-composite-images-tp3684430p3684434.html
Hi David,
The sample data has a 5 channel composite image. I am unsure how one
> creates such an image and how the colors are assigned. If you had 5
> grayscale images you wanted to merge to a composite and you want to
> designate the color of each channel, how would you do that? Thanks- Dave
>
First, make sure the five grayscale images are part of a single stack (Image
> Stacks > Images to Stack). Then convert it to a Composite image with:
Image > Color > Make Composite. By default, it will assign Red to channel
#1, Green to #2, Blue to #3, Cyan to #4 and Magenta to #5. (For #6 it's
Yellow and #7 is just black-to-white, i.e. Gray.)
To change the LUT for a particular channel, just change it using a command
such as Image > Lookup Tables > Fire. It will only affect the LUT for the
currently selected channel. There are shortcuts built in to the Channels
window that pops up when running "Make Composite"—just click the More button
to choose a LUT preset.
-Curtis
On Fri, May 27, 2011 at 3:03 PM, David Knecht <
[hidden email]>wrote:
> The sample data has a 5 channel composite image. I am unsure how one
> creates such an image and how the colors are assigned. If you had 5
> grayscale images you wanted to merge to a composite and you want to
> designate the color of each channel, how would you do that? Thanks- Dave
>
> Dr. David Knecht
> Department of Molecular and Cell Biology
> Co-head Flow Cytometry and Confocal Microscopy Facility
> U-3125
> 91 N. Eagleville Rd.
> University of Connecticut
> Storrs, CT 06269
> 860-486-2200
> 860-486-4331 (fax)
>