Re: multi-channel composite images

Posted by Michael P Ellis on May 28, 2011; 8:26am
URL: http://imagej.273.s1.nabble.com/multi-channel-composite-images-tp3684430p3684435.html

Dear Dr. David Knecht,

You may want to try my SmartCaptureLite extensions. After having turned your 5 greyscale images into a stack (as Curtis suggests) you can then use the CompositeAdjuster plugin to conveniently assign colours and adjust levels all from a single interactive palette. (Use the MakeComposite button on the CompositeAdjuster palette to convert your stack to a Composite image)

See http://www.dsuk.biz/DSUK/SmartCaptureLite.html and watch the movies to see how to assign colours to the individual planes.

The SmartCapturePlugin is free of charge and I would be interested receive any feedback.

Regards -- Michael Ellis

On 27 May 2011, at 21:44, Curtis Rueden wrote:

> Hi David,
>
> The sample data has a 5 channel composite image.  I am unsure how one
>> creates such an image and how the colors are assigned.  If you had 5
>> grayscale images you wanted to merge to a composite and you want to
>> designate the color of each channel, how would you do that?  Thanks- Dave
>>
>
> First, make sure the five grayscale images are part of a single stack (Image
>> Stacks > Images to Stack). Then convert it to a Composite image with:
> Image > Color > Make Composite. By default, it will assign Red to channel
> #1, Green to #2, Blue to #3, Cyan to #4 and Magenta to #5. (For #6 it's
> Yellow and #7 is just black-to-white, i.e. Gray.)
>
> To change the LUT for a particular channel, just change it using a command
> such as Image > Lookup Tables > Fire. It will only affect the LUT for the
> currently selected channel. There are shortcuts built in to the Channels
> window that pops up when running "Make Composite"—just click the More button
> to choose a LUT preset.
>
> -Curtis
>
> On Fri, May 27, 2011 at 3:03 PM, David Knecht <[hidden email]>wrote:
>
>> The sample data has a 5 channel composite image.  I am unsure how one
>> creates such an image and how the colors are assigned.  If you had 5
>> grayscale images you wanted to merge to a composite and you want to
>> designate the color of each channel, how would you do that?  Thanks- Dave
>>
>> Dr. David Knecht
>> Department of Molecular and Cell Biology
>> Co-head Flow Cytometry and Confocal Microscopy Facility
>> U-3125
>> 91 N. Eagleville Rd.
>> University of Connecticut
>> Storrs, CT 06269
>> 860-486-2200
>> 860-486-4331 (fax)
>>