Re: Automatically define and set particular regions of "grey" to 255

Posted by Nathaniel Ryckman on
URL: http://imagej.273.s1.nabble.com/Automatically-define-and-set-particular-regions-of-grey-to-255-tp3684565p3684567.html

I think everyone feels that their problem is quite simple ;-). Apparently, there aren't very good solutions to cellular image analysis from what I can tell.

A quick way to get a close approximation for what you want goes as follows:

1) Download the autothreshold package from Fiji.
2) Process -> Subtract background -> 50px and sliding paraboloid (Helps correct for noise/uneven lighting)
3) Image->Adjust->Autothreshold -> MinError(l) -> White objects... and SetThreshold instead of..
4) Image->Adjust->Threshold->Apply
5) Process->Noise->Despeckle
6) Edit->Invert
7) Analyze->Analyze Particles->700-Infinity (pixels) and add to manager
8) Select all your ROI's and click measure. You can export the table to an excel sheet from there.