Image subtraction, distance maps and skeletons to measure width

Posted by eburell on May 13, 2011; 7:33pm
URL: http://imagej.273.s1.nabble.com/Image-subtraction-distance-maps-and-skeletons-to-measure-width-tp3684590.html

Dear members,

I am trying to measure the thickness of a sponge spicule. I was told to dilate a binary image (from a HSB brightness slide) of the spicule and subtract the original to create a perimeter. In the next step, a distance map was to be created and I assume this can be done with the EDM plugin (I'm currently having problems with this plugin and I am unsure of what a proper output from using this would be). Finally, a skeleton of the spicule was to be created.

I am however not sure on how to calculate the distance from the skeleton to the perimeter using the distance map. Could anyone be so kind as to offer me some tips on this step?

One of the images I am working on can be found here: https://skitch.com/aiden/r9e7y/capture-3

Thanks in advance,
Eli