importing .LSM files into ImageJ and losing 1 channel

Posted by Holly Aaron on May 12, 2011; 9:43pm
URL: http://imagej.273.s1.nabble.com/importing-LSM-files-into-ImageJ-and-losing-1-channel-tp3684595.html

Dear ImageJ Community - Hello! It has been a long time since I've been on
the list. I am happy to see it is still so active!

Anyway, one of my users recently ran into an unusual problem with importing
files from the Zeiss 710 Zen software.  She is doing large tile scans (6x8
or bigger) z-stacks (20+ z- planes), so the files are 0.5G++.  She is doing
them in 2 rounds to get 3 colors w/o any cross-talk:  first GFP & Cy5 and
then Cy3.  She then adds the Cy3 channel to the other image to create a
3-channel image. She's saving in the .lsm format. All the data (all 3
channels) are there if she re-opens in Zen. But when she imports the file
into ImageJ, there are only the first 2 channels.  Does anyone know what
could be happening to the 3rd channel?  Are there limits to the file sizes
that can be opened?

Thanks for any advice or help!

-Holly
___________________________
Holly L. Aaron
Molecular Imaging Center
Cancer Research Laboratory
University of California Berkeley
251 LSA #2751
Berkeley, CA  94720-2751
510.642.2901
510.642.5741 fax
[hidden email]
http://imaging.berkeley.edu