Re: Reslice Problem

Posted by Rasband, Wayne (NIH/NIMH) [E] on May 12, 2011; 3:13pm
URL: http://imagej.273.s1.nabble.com/Reslice-Problem-tp3684610p3684611.html

On May 11, 2011, at 10:34 AM, Sebastian Rhode wrote:

> Hi,
>
> I recorded a z-stack of a 100nm bead with a voxel size of 64x64nm in
> XY and 50nm in Z dimension (image properties are correct). When I do
> the "Reslice" the dialog offers "Output Spacing = 50nm" which is fine.
> The resulting is then used to determine the PSF in Z direction.
>
> When I check the image properties of the resulting stack it still says
> 64 x 64 x 50nm, which is in my opion wrong. It should be 64 x 50 x
> 64nm, since i did a reslice.
>
> When you draw the a line profile along the z-axis of the PSF and check
> the list values, the 1st column contains 0nm, 64nm, 128nm, 192nm, ...
> But shouldn´t it be 0nm, 50nm, 100nm,  ...? The correct values are
> cruical if one wants to fit the coorect FWHM-Z for example.

You should be able to work around this problem by checking "Avoid interpolation" in the Image>Stacks>Reslice dialog box.

-wayne