Re: Determining Mean Fluorescence Intensity

Posted by aeyu on May 04, 2011; 4:11pm
URL: http://imagej.273.s1.nabble.com/Determining-Mean-Fluorescence-Intensity-tp3684719p3684720.html

Does anyone have concise instructions for determining the mean fluorescent intensity for RGB images.  I already got my ROI measurments for each color.  I am doing a ratio of green to red with 4 different experimental groups.

Mean/Area?

Do I normalize to anything?

Best!
dr^2mom