http://imagej.273.s1.nabble.com/3D-using-Stack-Focusser-tp3684866p3684868.html
> Do you mean you want to use something like stack focuser on the rotated
> stack?
>
> Mikhail Umorin, Ph.D.
>
>
> On Thu, Apr 21, 2011 at 2:23 PM, JOEL B. SHEFFIELD <
[hidden email]> wrote:
>
>> Colleagues,
>>
>> Here's an interesting challenge. As I understand it, both the 3D Viewer
>> or
>> the 3D rotation utility built into ImageJ work on some form of an
>> algorithm
>> in which the relative positions of the slices in a stack are shifted
>> relative to each other, and then a Maximum projection is generated,
>> creating
>> a new view through the shifted stack. We have been looking at the DIC
>> stacks that are generated either with confocal or manual DIC systems,
>> which
>> also have optical slices. However, when we try a standard Max projection,
>> the resultant is a mess, since the criteria for inclusion are not
>> intensity,
>> but contrast. On the other hand, we have been able to use the Stack
>> Focuser plugin to create "flattened" images of these stacks so that all
>> components remain in focus. I am wondering if it is possible to use an
>> image shift algorithm similar to the one for transparent fluorescent
>> objects
>> to generate a rotating, or at least a simple stereo view of such DIC
>> samples.
>>
>> --
>>
>>
>> Joel B. Sheffield, Ph.D
>> Department of Biology
>> Temple University
>> Philadelphia, PA 19122
>> Voice: 215 204 8839
>> e-mail:
[hidden email]
>> URL:
http://astro.temple.edu/~jbs>>
>
>