http://imagej.273.s1.nabble.com/3D-using-Stack-Focusser-tp3684866p3684869.html
No. I want to create a rotating image from a DIC stack, so I can see some
> Do you mean you want to use something like stack focuser on the rotated
> stack?
>
> Mikhail Umorin, Ph.D.
>
> On Thu, Apr 21, 2011 at 7:28 PM, Mikhail Umorin <
[hidden email]> wrote:
>
> > Do you mean you want to use something like stack focuser on the rotated
> > stack?
> >
> > Mikhail Umorin, Ph.D.
> >
> >
> > On Thu, Apr 21, 2011 at 2:23 PM, JOEL B. SHEFFIELD <
[hidden email]>
> wrote:
> >
> >> Colleagues,
> >>
> >> Here's an interesting challenge. As I understand it, both the 3D Viewer
> >> or
> >> the 3D rotation utility built into ImageJ work on some form of an
> >> algorithm
> >> in which the relative positions of the slices in a stack are shifted
> >> relative to each other, and then a Maximum projection is generated,
> >> creating
> >> a new view through the shifted stack. We have been looking at the DIC
> >> stacks that are generated either with confocal or manual DIC systems,
> >> which
> >> also have optical slices. However, when we try a standard Max
> projection,
> >> the resultant is a mess, since the criteria for inclusion are not
> >> intensity,
> >> but contrast. On the other hand, we have been able to use the Stack
> >> Focuser plugin to create "flattened" images of these stacks so that all
> >> components remain in focus. I am wondering if it is possible to use an
> >> image shift algorithm similar to the one for transparent fluorescent
> >> objects
> >> to generate a rotating, or at least a simple stereo view of such DIC
> >> samples.
> >>
> >> --
> >>
> >>
> >> Joel B. Sheffield, Ph.D
> >> Department of Biology
> >> Temple University
> >> Philadelphia, PA 19122
> >> Voice: 215 204 8839
> >> e-mail:
[hidden email]
> >> URL:
http://astro.temple.edu/~jbs> >>
> >
> >
>
Joel B. Sheffield, Ph.D