Re: soma area detection

Posted by Qing Liu on Apr 18, 2011; 10:39pm
URL: http://imagej.273.s1.nabble.com/soma-area-detection-tp3684897p3684898.html

Dear all, I have found a solution to select neuronal soma but need your
suggestions to do so in imageJ.

http://dl.dropbox.com/u/266880/Soma.pdf

In the above paper, they mentioned

the images of the neurons stained
> for beta-III tubulin are first Gaussian filtered to suppress image
> noise. They are background corrected using a top hat morphological filter
> with a disk structuring element larger than the
> size of the largest cell body. A morphological opening
> with a structuring element of a diameter smaller than the cell
> body but larger than all neurites permits suppressing neuritelike
> structures.
>

I am a little confused about the second and third step and wonder if anyone
can help.

Thank you.

Qing