effect of magnification on area measurements

Posted by Cheuk Tam on Apr 15, 2011; 5:54pm
URL: http://imagej.273.s1.nabble.com/effect-of-magnification-on-area-measurements-tp3684968.html

Dear ImageJ Community,

I am new to ImageJ and am using it to measure the area of DAB-stained
cells.  I tried measuring the area of the same cell at different
magnifications (and set the scale to the respective stage micrometer
images), but I found the area measurements yielded different numbers.  The
40X objective had an area that was about 4-fold smaller than the 10X
objective image.  Is this because the resolution and the number of pixels in
each image are different?  If so, which area measurement is the accurate
one?   Thank you for your help.

-Cheuk