Posted by
pang on
Apr 15, 2011; 7:47pm
URL: http://imagej.273.s1.nabble.com/effect-of-magnification-on-area-measurements-tp3684968p3684970.html
You need to use Analyze/Set Scale function of the ImageJ. As long as you
set the scale correctly, you should get the same area for two images at
different magnification. ImageJ itself does not know the scale unless
you set it.
I suspected that you use the Image/Scale function, this will
reduce/enlarge your image.
Zhengyu Pang, Ph.D.
Biochemistry and Biological Engineering Laboratory
Diagnostic and Biomedical Technologies
GE Global Research, K1-5B37A
Niskayuna, NY 12309
T: 518-387-4015
F: 518-387-7765
-----Original Message-----
From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of
Cheuk Tam
Sent: Friday, April 15, 2011 1:54 PM
To:
[hidden email]
Subject: effect of magnification on area measurements
Dear ImageJ Community,
I am new to ImageJ and am using it to measure the area of DAB-stained
cells. I tried measuring the area of the same cell at different
magnifications (and set the scale to the respective stage micrometer
images), but I found the area measurements yielded different numbers.
The 40X objective had an area that was about 4-fold smaller than the 10X
objective image. Is this because the resolution and the number of
pixels in each image are different? If so, which area measurement is
the accurate
one? Thank you for your help.
-Cheuk