Re: effect of magnification on area measurements

Posted by Nathaniel Ryckman on Apr 15, 2011; 6:16pm
URL: http://imagej.273.s1.nabble.com/effect-of-magnification-on-area-measurements-tp3684968p3684972.html

"and set the scale to the respective stage micrometer images"

What do you mean by that statement? Just to check, do you mean that you changed the scale for each image that you were looking at by using Analyze->Set Scale?

Could you provide us with the settings you used for each image?

Cheuk Tam wrote

Dear ImageJ Community,

I am new to ImageJ and am using it to measure the area of DAB-stained
cells.  I tried measuring the area of the same cell at different
magnifications (and set the scale to the respective stage micrometer
images), but I found the area measurements yielded different numbers.  The
40X objective had an area that was about 4-fold smaller than the 10X
objective image.  Is this because the resolution and the number of pixels in
each image are different?  If so, which area measurement is the accurate
one?   Thank you for your help.

-Cheuk