JACoP parameter and PSF

Posted by gauri on Mar 11, 2011; 4:03pm
URL: http://imagej.273.s1.nabble.com/JACoP-parameter-and-PSF-tp3685080.html

Hi,
I am looking at colocalization of two proteins on confocal microscope. I am using JACoP plugin to find Pearson
's coefficient and mander's coefficient. I had a few questions about setting parameters for JACoP. For the microscope parameter it asks about XY calibration and Z calibration. I am not sure what does that mean and what will be the correct value for it. The other problem i had was does JACoP calculate PSF. What PSF does it use. it never asks anythiong about PSF.
Thanks
Gauri