automatic detection out-of-focus images

Posted by Crowell Elizabeth on Mar 11, 2011; 6:01pm
URL: http://imagej.273.s1.nabble.com/JACoP-parameter-and-PSF-tp3685080p3685081.html

Hello fellow ImageJ users,

Has anyone developed an algorithm in ImageJ for determining if an image
is out-of-focus?

I managed to flag images that are likely to be out-of-focus based on
intensity measurements of DAPI (nuclear) staining after running Find
Edges and thresholding, but any method based on intensity seems to fail
from one experiment to the next, since the global intensity varies
depending on staining success and lamp power, etc.  I did not find any
plugins or macros on the ImageJ site that present such a screening
procedure.

I'm sure someone out there must know of a way to do this well, since it
is a common problem!

Kind Regards,

 

Elizabeth CROWELL

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