Re: automatic detection out-of-focus images

Posted by vtali on Apr 07, 2011; 7:03pm
URL: http://imagej.273.s1.nabble.com/JACoP-parameter-and-PSF-tp3685080p3685082.html

We had the same problem with waterborne pathogen detection using FTIC/DAPI florescense channels and for Whole Slide Imaging of histology samples (H&E).

The key issue is that it is not the whole Image (Field of View) that is out of focus but some parts of it, because sample of "thick" for the objective focus depth .

We have tried avaialbe ImageJ plugings from here but none of tham

Now we are tying to find approach and write Java code for deconvolution that would work adaptevely.
The idea is to use Z-stack on representative VOF with known Z movement; reconstruct PSF and use it adaptevely across the image.

I will send update if you come up with something.

One Tip that we found: Adaptive background removal (Rolling ball in ImageJ)  + Unsharp helps with segmentation.

I too would be very interested if somebody finds a way to adaptively deconvolve focus blur on "thick" samples.

Vitali
Smart Imaging Technologies
713-589-3500
live.simagis.com