Re: automatic detection out-of-focus images

Posted by dscho on Apr 08, 2011; 12:40am
URL: http://imagej.273.s1.nabble.com/JACoP-parameter-and-PSF-tp3685080p3685083.html

Hi,

On Thu, 7 Apr 2011, vtali wrote:

> We had the same problem with waterborne pathogen detection using
> FTIC/DAPI florescense channels and for Whole Slide Imaging of histology
> samples (H&E).
>
> The key issue is that it is not the whole Image (Field of View) that is
> out of focus but some parts of it, because sample of "thick" for the
> objective focus depth .
>
> We have tried avaialbe ImageJ plugings from here but none of tham

... yes?

The bigger issue is: how do _you_ differentiate between out-of-focus and
naturally blurry parts of an image?

Hth,
Johannes