Posted by
Daniel James White on
Mar 12, 2011; 7:29am
URL: http://imagej.273.s1.nabble.com/JACoP-parameter-and-PSF-tp3685080p3685091.html
Hi Elizabeth,
On Mar 12, 2011, at 6:08 AM, IMAGEJ automatic digest system wrote:
>
> Date: Fri, 11 Mar 2011 19:01:24 +0100
> From: Crowell Elizabeth <
[hidden email]>
> Subject: automatic detection out-of-focus images
>
> Hello fellow ImageJ users,
>
> Has anyone developed an algorithm in ImageJ for determining if an image
> is out-of-focus?
yes, several.
See
http://pacific.mpi-cbg.de/wiki/index.php/Extended_Depth_of_Field>
> I managed to flag images that are likely to be out-of-focus based on
> intensity measurements of DAPI (nuclear) staining after running Find
> Edges and thresholding, but any method based on intensity seems to fail
> from one experiment to the next, since the global intensity varies
> depending on staining success and lamp power, etc.
Yes, thats the case.
> I did not find any
> plugins or macros on the ImageJ site that present such a screening
> procedure.
http://pacific.mpi-cbg.de/wiki/index.php/Extended_Depth_of_Field>
> I'm sure someone out there must know of a way to do this well, since it
> is a common problem!
its been worked on a lots...
so you should be able to use ideas from
http://pacific.mpi-cbg.de/wiki/index.php/Extended_Depth_of_Fieldhttp://en.wikipedia.org/wiki/Focus_stackingcheers
Dan
>
> Kind Regards,
>
>
>
> Elizabeth CROWELL
Dr. Daniel James White BSc. (Hons.) PhD
Senior Microscopist / Image Processing and Analysis
Light Microscopy Facility
Max Planck Institute of Molecular Cell Biology and Genetics
Pfotenhauerstrasse 108
01307 DRESDEN
Germany
+49 (0)15114966933 (German Mobile)
+49 (0)351 210 2627 (Work phone at MPI-CBG)
+49 (0)351 210 1078 (Fax MPI-CBG LMF)
http://www.bioimagexd.net BioImageXD
http://pacific.mpi-cbg.de Fiji (is just ImageJ - batteries included)
http://www.chalkie.org.uk[hidden email]
(
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