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Re: split channels and all files in a directory

Posted by Ramon Guirado on Apr 07, 2011; 4:54pm
URL: http://imagej.273.s1.nabble.com/split-channels-and-all-files-in-a-directory-tp3685101p3685103.html

Thanks for this macro, I think many people share the hate for imageJ macro
language, indeed would be better to learn python instead.
Anyway I think I can find all the commands I need in your macro, just it
will require time to decipher what is what I need.
Thanks!!

ramon

2011/4/7 Carnë Draug <[hidden email]>

> On 7 April 2011 15:04, Ramon Guirado <[hidden email]> wrote:
> > Hi, I'm trying to write a macro able to open all the files in a
> directory,
> > split the channels of the images and analyze particles in this separated
> > channels as well as the composite between some of them (they will be also
> > binarized, etc...). I have just done small macros using the macro
> recorder,
> > so I don't know how to do it, I'm trying to get the identity of every
> image
> > in a directory and then I guess after the image calculator, I will have
> to
> > identify the new windows just created so they are processed as well, and
> > then do that recursively, but I feel a little bit lost... which would be
> the
> > easiest way to proceed? by know I'll try to understand the macro posted
> > by Carnë Draug..
> >
> > Thanks,
> >
> >
> > ramon
>
> I wrote a macro some time ago that does a similar thing. It opens all
> images, makes them composite, use one channel to find the nucleus
> (DAPI stained), and the two other channels to analyze particles inside
> the nucleus. I wrote it as macro and regret it, it's terribly
> confusing code. I find the ImageJ macro language terribly frustating.
> I should have bothered to learn Python to do it. Anyway, I pasted it
> into pastebin
>
> http://pastebin.com/R5dPsyqM
>
> You'll also need the other functions I posted before (I place them in
> macros/Library.txt so I don't have to bother copying into every
> macro).
>
> Carnë
>