ICA for Triple Labelling

Posted by alexa_11 on Apr 05, 2011; 4:19pm
URL: http://imagej.273.s1.nabble.com/ICA-for-Triple-Labelling-tp3685137.html

Hi,

I am attempting to find a method of retrieving an ICQ for three channels using the Intensity Correlation Analysis plugin. One of these channels is a marker for synapses, and I'm interested in looking at the colocalization of the other two proteins with this marker.

This might be a bit far fetched, but I was wondering if it would make sense to use the +PDM image (with all the negative pixel images removed since they are blank in my case, and crop the extra black strip added to the bottom so the size corresponds with channel 3) and run it through the ICA program with a third channel. I know it works, but I'm not sure if it makes sense to take the ICQs as given.

Thank you,

Alex