Posted by
Daniel James White on
Apr 06, 2011; 10:30am
URL: http://imagej.273.s1.nabble.com/ICA-for-Triple-Labelling-tp3685137p3685138.html
Hi Alex,
On Apr 6, 2011, at 6:00 AM, IMAGEJ automatic digest system wrote:
>
> Date: Tue, 5 Apr 2011 09:19:57 -0700
> From: alexa_11 <
[hidden email]>
> Subject: ICA for Triple Labelling
>
> Hi,
>
> I am attempting to find a method of retrieving an ICQ for three channels
> using the Intensity Correlation Analysis plugin.
then you need to do each channel pair individually,
so thats 3 tests.
> One of these channels is a
> marker for synapses, and I'm interested in looking at the colocalization of
> the other two proteins with this marker.
>
> This might be a bit far fetched, but I was wondering if it would make sense
> to use the +PDM image (with all the negative pixel images removed since they
> are blank in my case, and crop the extra black strip added to the bottom so
> the size corresponds with channel 3) and run it through the ICA program with
> a third channel. I know it works, but I'm not sure if it makes sense to take
> the ICQs as given.
Can you imagine or write out how this might make sense mathematically?
If you can, then please share.
If you can not, then you are not doing something that might give any meaningful results?
Think about how you are framing your question.
Coloc as defined by the ICQ method of Li
is able to compare 2 images, not three or more.
you can talk only about the correlation between pairs of image data sets.
I am not sure sure what toy are trying to achieve???
Can be explain in more details what you are trying to measure exactly?
cheers
Dan
>
> Thank you,
>
> Alex
Dr. Daniel James White BSc. (Hons.) PhD
Senior Microscopist / Image Visualisation, Processing and Analysis
Light Microscopy and Image Processing Facilities
Max Planck Institute of Molecular Cell Biology and Genetics
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