Re: ICA for Triple Labelling

Posted by alexa_11 on Apr 06, 2011; 3:11pm
URL: http://imagej.273.s1.nabble.com/ICA-for-Triple-Labelling-tp3685137p3685139.html

Hi Dan,

I am looking at two proteins that localize to synapses however they can also be located elsewhere, for this reason I need to compare with the synaptic marker.

I already analysed the images as pairs x3 but it would be great to see in which cases protein A colocalizes with protein B in the synapses. With the analysis of each pair you are able to see how often protein A or B is localized to synapses, and how often protein B colocalizes with protein A, but you are not able to quantify the three as a whole.

The way I understand it, the +PDM image gives you the pixels which colocalize in a dependent manner (in this case giving me the population of protein A that localize to synapses).

Now, I'm not sure if the pixel intensities in the PDM image would be of any meaning when comparing with protein B. And mathematically, I'm also not sure if this makes sense.

Thanks,

Alex