Re: Watershed Algorithm source - bug

Posted by Nathaniel Ryckman on Mar 30, 2011; 4:51pm
URL: http://imagej.273.s1.nabble.com/Watershed-Algorithm-source-bug-tp3685192p3685197.html

If you are able to make something that is effective, please keep us updated :-)!

I also was interested in Daniel Sage's program, but the program took way too long to run. In addition, I have a very amorphous cell set to work with (macrophages from drosophila that are often clumped and always unevenly stained), and his program wasn't precise enough for use.

Even on the demo picture on the page, the software doesn't do a good enough job of segmentation:
http://bigwww.epfl.ch/sage/soft/watershed/

I created a large macro that uses the grayscale image to segment nuclei. The program currently works excellently. The only glaring problem is that the program will obtain ROI's that are too small when the blobs are heavily clumped and have very similar areas of high intensity pixels touching each other.

Unfortunately, I think that I too will have to resort to editing the watershed program since the macro language doesn't offer enough control as far as I am aware of.